FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n02_AH9723I-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n02_AH9723I-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427676
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC8900.032.59857644
GTCGCCG9800.027.7675945
AGATCGG12400.027.2821661
CGTGTAG11300.026.87808416
AGCGTCG12600.026.78352511
GTCGTGT12050.026.69906214
GCGTCGT12500.026.63708712
TGGTCGC10800.026.44763643
GATCGGA13200.025.7769282
CGTCGTG12800.025.662213
GTGGTCG11450.025.33956342
CTCGGTG10100.024.94201738
GTGTAGG12350.024.59313217
GAGCGTC14400.023.28020510
ATCTCGG10650.023.23368336
AGAGCGT14700.023.1127019
GTAGGGA13050.023.1009219
AAGAGCG15050.022.5776948
TGTAGGG13500.022.49753818
TCGTGTA15150.021.23569115
Produced by FastQC (version 0.11.5)