FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n02_AH9722I-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n02_AH9722I-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7442369
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG113730.1528142450340745Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC19650.037.5420944
GTCGCCG19900.035.6009645
CGTGTAG22550.035.51579316
GCGTCGT24650.035.5023112
AGCGTCG25200.034.81743611
CTCGGTG18700.033.79900738
GTGGTCG22900.033.5899642
GTCGTGT26000.032.79387314
GTGTAGG24450.032.75631717
GTAGGGA24700.032.2421819
TGTAGGG25100.032.08730318
ATCTCGG19700.032.08526636
AGATCGG27800.032.0512961
GGTGGTC24350.030.66667641
GATCTCG20500.030.50397935
TCGTGTA28700.029.70833615
AAGAGCG30350.029.4345868
TGGTCGC25300.029.42527243
GATCGGA30600.028.872822
TCGGAAG30550.028.5356314
Produced by FastQC (version 0.11.5)