FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n02_AH8643I2-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n02_AH8643I2-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5567962
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG444310.797975991933853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG12850.09.27716738
TTAGGGG10200.07.27726337
TAGGGTG6803.027526E-86.94629238
TTGGGGG43050.06.47874938
TGGGGCG6555.656939E-66.18043745
TTTGGGG29200.06.162564837
GGGTTGT19050.05.43156530
GTGGGCG5500.0053118155.315800745
AGGGGGG49250.05.297785839
TAAGGGG8102.9755405E-55.27607838
ATGGGGG15501.4551915E-115.224123538
GGGGGAT16403.6379788E-125.21248521
GGGGTTT27800.05.178467329
GGGGAGA14352.6375346E-105.17329322
GGGGGTA18100.05.09577622
GTAGGGG11053.4272853E-75.08899637
GGGAGTG11651.9937397E-75.020473525
Produced by FastQC (version 0.11.5)