FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n02_AH7797I-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n02_AH7797I-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15485623
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA643550.41557901803498637No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC551650.3562336497537103No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236580.15277396330777263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGG66750.039.30002216
CCTCGGG67450.038.65880615
TCGGGGA86900.038.49762317
GGCGGTC87250.038.33439638
TAAGCCG85500.037.28629716
CCAGGCG90100.036.92473235
CTGACCC85250.036.7521740
GTCTCCC91150.036.74117742
CAGGCGG91550.036.41326536
CGGTCTC92850.036.28742640
CCAGGGA88300.036.1338355
CCCAGGC92000.036.04002434
GCGGTCT94050.035.73015639
AGGCGGT95100.035.19556437
GGTCTCC95650.035.13099741
CCTGACC89450.034.927339
AGCCGAT92300.034.7339818
TCCAGGG92100.034.7045484
GACGTCA92350.034.58989325
TCCTCGG76450.034.22545214
Produced by FastQC (version 0.11.5)