FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n02_AH7797A-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n02_AH7797A-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8973310
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166010.18500419577614058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGG4700.012.44542916
GGCGGTC6250.012.235846538
CCTCGGG5100.011.02831415
CCAGGCG8550.09.99772235
TCGGGGA7200.09.68637317
CCCAGGC9700.09.27623734
GCGGTCT9650.08.623919539
CAGGCGG9200.08.55778536
GTCTCCC10000.08.09666442
CGGTCTC10100.07.57139140
TAAGCCG7852.3646862E-117.45140316
CTGACCC7801.6007107E-107.208810340
TCCTCGG10600.06.791813414
AGGCGGT12150.06.479751637
CCTGACC7704.7170033E-86.426318639
TCCCAGG15000.05.848699633
GGTCTCC13701.2732926E-115.581797641
CGGGGAA14304.3655746E-115.34896818
CCAGGGA11056.046321E-85.29819875
AGCCGAT14058.0035534E-105.12390218
Produced by FastQC (version 0.11.5)