FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5727127
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106640.1862015631921555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG34000.038.22579611
ACACGTC33550.037.42226813
CACACGT34900.037.11247312
CACGTCT34400.036.3035914
ACGTCTG34950.036.1220515
CGTCTGA34950.035.96818516
AGCACAC37100.035.930510
AGAGCAC38650.035.361988
GATCGGA38750.035.295071
GAACTCC36150.034.6335221
TCGGAAG40850.034.518463
ATCGGAA42700.033.3924832
CGGAAGA43700.032.164614
CCAGTCA31850.031.78951526
CTCCAGT37350.031.2600224
TGAACTC41600.030.53110320
CAGTCAC29450.030.24952927
CTGAACT43600.028.63012119
TCCAGTC37900.028.07840225
GAGCACA49850.027.642539
Produced by FastQC (version 0.11.5)