FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_AH9724I-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH9724I-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5852894
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG12300.021.9614431
GCACACG12800.020.91698512
ACACGTC13300.020.1306314
CACGTCT14250.019.10436415
ACGTCTG14100.018.9886316
CACACGT14700.018.21342813
GATCGGA15850.017.4651172
CAGTCAC16300.016.56363728
AGCACAC16050.016.54127711
GAGCACA16300.016.28757710
GAACTCC16600.015.9933622
CGTCTGA17200.015.69706917
TCCAGTC17400.015.25790226
AGTCACA17900.015.20878129
CCAGTCA17650.015.16925727
GTCACAT18450.014.51151230
TCGGAAG18550.014.3162754
ACTCCAG18650.014.11461524
GTCTGAA19700.014.04768318
TATCTCG13600.013.73108942
Produced by FastQC (version 0.11.5)