FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_AH9723I-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH9723I-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427676
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT63660.1172877673612058TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG14050.024.6705781
CACGAGT16900.020.10282732
AGTCACG17850.019.28501929
ACACGTC17550.019.23007614
GCACACG18100.019.01865212
GTCACGA18000.018.87432130
ACGTCTG18950.018.16590716
GATCGGA19250.017.8864272
CACGTCT19500.017.7685915
CACACGT19400.017.74420513
ACGAGTG19600.017.67793533
TCACGAG19800.017.38573831
CGTCTGA20200.017.04177917
CGAGTGG20650.016.56114834
TCGTATG15600.016.2974945
AGCACAC21200.016.02536611
TATCTCG19800.015.90851842
CAGTCAC21850.015.85755328
TCCAGTC22500.015.49944126
GAGCACA22250.015.47135310
Produced by FastQC (version 0.11.5)