FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_AH9723A-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH9723A-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6209181
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG93790.1510505169683409TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG25550.033.90277511
CCGTACG25450.033.32874733
ACGTCTG27350.031.67151315
CGTACGT26950.031.30674734
ACACGTC28000.031.09698913
CACGTCT27950.030.99162514
CACACGT28700.030.5737112
CGTCTGA29050.030.282816
GATCGGA28650.029.854741
AGCACAC30350.028.98591210
GAACTCC30300.028.43947621
GTACGTA30550.028.13309735
CAGTCAC31500.027.49891727
ACCGTAC31650.027.22641832
GTCACCG31800.026.88573529
CCAGTCA32600.026.84710326
GAGCACA33500.026.5290189
TCCAGTC33100.026.23763825
AGTCACC33050.026.07310328
AGAGCAC34000.025.9403638
Produced by FastQC (version 0.11.5)