FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_AH9722I-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH9722I-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7442369
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC153300.20598279929414948TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC112110.15063751877930268TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG26900.032.6320841
GTATGCC20400.029.55790745
GCACACG37100.023.71220212
AGTCACG38100.022.97172729
GTCACGA38450.022.7626230
ACGTCTG39000.022.73021516
CACGTCT38950.022.70147715
CGATCAG38600.022.67416834
ACACGTC39000.022.67237314
CACACGT39350.022.3563613
AGCACAC40350.021.9695811
GATCTCG36100.021.56442640
CACGATC41400.021.35803432
TCACGAT41200.021.29788231
GAGCACA41550.021.22677610
CCAGTCA41250.021.21752427
TCCAGTC41400.021.19499626
CAGTCAC41500.021.08970628
GAACTCC42400.020.69525122
TCGGAAG44500.019.924614
Produced by FastQC (version 0.11.5)