FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n01_AH9041I-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH9041I-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15215308
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1904201.2515027628753883TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG259350.042.8121611
GATCGGA257750.042.4585531
CACACGT263750.042.05529412
ACACGTC263400.041.98304713
ACGTCTG262400.041.91153715
AGTCACG262550.041.84474628
CACGTCT264800.041.65062714
GTCACGT264150.041.50610729
AGCACAC270000.041.3067810
GATCTCG228350.041.23449341
CGTCTGA267150.041.1917416
CGATCTC228550.041.02121440
GAGCACA271850.040.9844329
AGAGCAC274100.040.689058
CACGTGA270050.040.63261831
CAGTCAC270800.040.55331427
TCCAGTC269650.040.52601225
TCACGTG271550.040.37502730
TCGGAAG277250.040.37443
CCAGTCA271650.040.19451526
Produced by FastQC (version 0.11.5)