FastQCFastQC Report
Thu 12 Jul 2018
H3TN2AFXY_n01_AH8643I2-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH8643I2-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5567962
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG553970.9949241751290687TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTATG222610.39980517108414176TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGGATG95720.17191209279086317TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTTTG68040.12219910983587892TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGGATG64740.11627234524948266TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATT60620.10887286946283038TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG218350.043.96832311
GATCGGA216450.043.8514751
ACACGTC220800.043.5415913
CACACGT221750.043.365212
CACGTCT221650.043.2934114
ACGTCTG221650.043.24265715
AGCACAC223150.043.15363310
CGTCTGA222550.043.08799716
TCGGAAG225900.043.0233043
GAGCACA224300.042.9227379
AGAGCAC225900.042.7183238
GTTCCGT218550.042.6309435
AGTTCCG221150.042.49634
GTCACAG224150.042.47968329
ATCGGAA229300.042.4446262
CAGTCAC224450.042.44295527
AGTCACA224950.042.41863328
CCGTATC206800.042.38760438
TCCAGTC223950.042.3669225
GAACTCC225600.042.1863321
Produced by FastQC (version 0.11.5)