FastQCFastQC Report
Thu 12 Jul 2018
H3TN2AFXY_n01_AH8643I1-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH8643I1-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7818844
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC639040.8173075201398058TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC91200.042.60547329
GCACACG93500.042.2552611
GATCGGA93250.041.8806081
AGTCACG93450.041.84449828
ACACGTC95500.041.4174513
TCACGCC94100.041.26853630
CACACGT96050.041.15686412
ACGTCTG96150.041.1140615
CACGCCA95600.040.76222631
CGTCTGA97050.040.6400516
CACGTCT97450.040.61176714
AGCACAC98500.040.24737510
GAGCACA100600.039.608519
CAGTCAC99400.039.38499527
TCGGAAG101650.039.384753
TCCAGTC99850.039.1849625
GTATGCC84450.038.84416645
CCAGTCA101100.038.70047826
CGCCAAT101100.038.5669533
TATCTCG90200.038.56300439
Produced by FastQC (version 0.11.5)