FastQCFastQC Report
Thu 12 Jul 2018
H3TN2AFXY_n01_AH8643A2-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH8643A2-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9098094
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG671000.7375171107267082TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG97050.040.73223511
GATCGGA96350.040.581211
ACACGTC99450.039.88513
CGTCTGA100000.039.41835816
CACGTCT100800.039.32850614
AGCACAC101300.039.24543410
CACACGT101000.039.22834812
ACGTCTG100650.039.20828615
GAGCACA105000.037.9912769
CAGTCAC104850.037.57333427
GAACTCC104250.037.48764421
CACAGTC104800.037.41951831
AGAGCAC107750.037.230478
TCGGAAG108650.037.0989463
GTCACAG106450.036.9663229
TCCAGTC106650.036.77062225
ATCTCGT93600.036.6569342
CCAGTCA107200.036.6239426
AGTCACA108150.036.53087228
GTCTGAA108050.036.4815917
Produced by FastQC (version 0.11.5)