FastQCFastQC Report
Thu 12 Jul 2018
H3TN2AFXY_n01_AH8038I-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH8038I-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6000568
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC666571.1108448400218112TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG93600.042.546411
ACACGTC94450.042.18767513
GATCGGA93300.042.024451
ACGTCTG94850.041.9148815
CACACGT95200.041.78406512
CACGTCT95750.041.59139314
CGTCTGA96600.041.31893516
AGCACAC96450.041.31252310
GTATGCC89650.040.8070945
GAGCACA98100.040.6635369
ACTGACC95700.040.57833532
TCCAGTC97950.040.42719325
TCGGAAG99950.040.3486673
CGTATGC91050.040.13021544
AGAGCAC100100.040.0983168
CAGTCAC99400.040.0638127
CCAGTCA99250.039.98834626
AGTCACT100100.039.55887628
GTCTGAA101400.039.4739517
GAACTCC100850.039.465821
Produced by FastQC (version 0.11.5)