FastQCFastQC Report
Thu 12 Jul 2018
H3TN2AFXY_n01_AH7797K-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH7797K-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17395710
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC295920.1701109066545717TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCT48250.035.15941231
TCACGGC50050.033.76007530
GTCACGG51100.033.15443429
GCACACG51650.033.01918811
AGTCACG51300.032.893628
ACACGTC53700.031.75868213
GATCGGA53850.031.1397691
CACACGT55150.030.96448112
CGGCTAC55500.030.48543433
ACGTCTG57050.029.6966115
CACGTCT58350.029.22778514
ACGGCTA58550.029.12794532
CATCTCG51600.028.47285539
CGTCTGA60750.028.18421416
GTATGCC54400.028.1241145
AGCACAC61400.027.95913310
GAACTCC63550.026.87161321
GGCTACA61350.026.7716934
CGTATGC58500.026.2299444
CAGTCAC65650.026.08059327
Produced by FastQC (version 0.11.5)