FastQCFastQC Report
Thu 12 Jul 2018
H3TN2AFXY_n01_AH7797J-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH7797J-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4189998
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG27600.039.78156311
ACACGTC28450.038.67209613
ACGTCTG28750.038.58159615
GATCGGA28300.038.1738431
CACACGT28800.038.12412
CACGTCT29450.037.28255514
AGCACAC30600.036.2490510
GAGCACA31100.035.8837289
CGTCTGA31300.035.22271716
TATCGGG17250.035.21646539
CAGTCAC31450.035.12626327
CACTAGC31800.035.0934231
GAACTCC31650.034.83320621
AGAGCAC31950.034.7882358
TCACTAG32150.034.50142730
TCCAGTC32500.034.19910425
ACTAGCT32700.033.92112732
TAGCTTA32700.033.71471434
AGTCACT33050.033.56190528
TCGGAAG33450.033.5024763
Produced by FastQC (version 0.11.5)