FastQCFastQC Report
Thu 12 Jul 2018
H3TN2AFXY_n01_AH7797I-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH7797I-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15485623
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG984710.6358865897742699TruSeq Adapter, Index 16 (97% over 40bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA718780.46415956271181347No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC535530.34582399429457894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG143900.042.6061835
GTCCCGA139050.042.0211436
ACCCGTC149050.041.25480732
CCCGTCC150650.041.1302833
TCACCCG150000.040.97852330
GCACACG155350.040.9576511
CACCCGT151000.040.73694631
ACACGTC157050.040.7005413
ACGTCTG157000.040.6848415
CCGTCCC152000.040.45413234
CACACGT157850.040.3517312
CCCGATC126000.040.23069438
GATCGGA157650.040.105261
CTCGGGG63800.040.06125616
CGTCTGA159300.039.94206216
CACGTCT159850.039.91723614
TCCCGAT139000.039.30074337
GGCGGTC81400.039.0833638
TCGGGGA81650.038.9361417
GTCACCC159050.038.9014629
Produced by FastQC (version 0.11.5)