FastQCFastQC Report
Thu 12 Jul 2018
H3TN2AFXY_n01_AH6978I-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH6978I-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4896302
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG12550.026.89158211
CTTAGGC12700.026.57396333
ACTTAGG12650.026.32328232
GATCGGA12700.025.8735161
ACACGTC13350.024.94303313
CACACGT14250.023.68346212
ACGTCTG13950.023.54764415
CACGTCT14150.023.37382114
AGCACAC15100.022.20128610
CAGTCAC14600.022.19108227
CGTCTGA14950.021.97255116
CCAGTCA16400.020.85326
AGAGCAC16600.020.1953538
GAGCACA16500.020.1811819
AGTCACT16850.019.6284628
TTAGGCA17100.019.60464534
TCCAGTC16850.019.49493425
TAGGCAT16700.018.72695435
GAACTCC17700.018.68585221
CACTTAG18050.018.44817231
Produced by FastQC (version 0.11.5)