FastQCFastQC Report
Thu 12 Jul 2018
H3TN2AFXY_n01_AH6978A-0718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n01_AH6978A-0718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7778934
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC88420.11366595988602038TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG23950.023.9650971
GCACACG27750.020.7559612
GTATGCC17550.020.25563245
CACACGT28650.019.94687813
ACACGTC29250.019.5377114
ACGTCTG29250.019.46091716
GATCGGA29200.019.422182
CACGTCT29700.019.31744215
CGTCTGA30700.018.76161417
GATCTCG24650.018.52869840
AGCACAC31650.018.41161511
CGATCTC24850.018.37957439
CAGTCAC32150.018.05529428
CATCACG31600.017.87114734
CACGATC26100.017.75793837
GAGCACA33150.017.5785110
TCCAGTC32950.017.34379626
CGTATGC32100.017.3124245
AGTCACA34050.016.9156529
CCAGTCA34000.016.87435227
Produced by FastQC (version 0.11.5)