FastQCFastQC Report
Tue 3 Jul 2018
H3KCTBGX7_n01_nlp7_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9046915
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1243641.374656443660629No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC427330.4723488614627196TruSeq Adapter, Index 9 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG320120.3538443767847935No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG212460.23484248497968643No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC197370.21816276598155285No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG182490.20171517030943698No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG153070.16919579768351975No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA152950.16906315578293815No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC144630.15986665067594866No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA139310.15398619308349865No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT138710.1533229835805907No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG128260.14177208473827818No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT126470.13979350972126964No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC126080.1393624235443795No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA125720.13896449784263476No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT116700.12899424831558604No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC115850.12805470151979984No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG109670.1212236436398485No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG105780.11692383536266229No Hit
CCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAA102890.11372937625698926No Hit
TCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAG101300.11197187107428333No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG101210.11187238964884715No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAA97780.10808104199055699No Hit
CCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAG97030.10725203011192214No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA96740.10693147885218332No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA95820.10591455761439121No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT93800.10368175228793461No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA90660.10021095588938328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG55450.061.28825446
CGTATGC56000.060.74814644
TCGTATG56050.060.5066343
GTATGCC56450.060.26387845
CTCGTAT54450.059.58495742
ATGCCGT59100.057.44452347
AGTCACG62200.054.974228
GCCGTCT65400.051.6432949
GTCACGA66200.051.54676429
CAGTCAC67350.051.34215527
CTGCTTG66450.051.30158257
CCGTCTT66350.050.69286350
AGATCTC62150.050.68340338
AAAAAGG63350.050.60618669
TCAGATC62400.050.48034336
CTGAACT69350.050.41774419
ACGTCTG69900.049.92006715
TCCAGTC70450.049.0829625
CACGTCT71350.049.0042214
GTACTCT207100.048.7986563
Produced by FastQC (version 0.11.5)