H3KCTBGX7_n01_nlp7_5.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3KCTBGX7_n01_nlp7_5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9597538
Sequences flagged as poor quality	0
Sequence length	76
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT	148515	1.5474281008316926	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	44486	0.46351470554219215	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC	29114	0.3033486296173039	TruSeq Adapter, Index 5 (100% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	24175	0.25188751531903286	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	22316	0.23251796450297985	No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA	22054	0.22978809773923273	No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG	21938	0.22857945443925304	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG	21170	0.22057740224628442	No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT	16731	0.1743259573444773	No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC	16447	0.17136686512728577	No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG	14525	0.1513408959672783	No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC	14328	0.14928828622507148	No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA	14021	0.1460895492156426	No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA	13688	0.14261990939759758	No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC	12654	0.13184631308571013	No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT	12552	0.13078354052883145	No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG	12398	0.12917896235472057	No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG	12224	0.1273659974047511	No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT	12157	0.12666790170562492	No Hit
CCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAA	11645	0.12133320024364583	No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA	10823	0.11276850375585906	No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG	10689	0.11137231235760672	No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC	10580	0.11023660442917756	No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT	9978	0.10396416247583494	No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG	9949	0.10366200165084004	No Hit
GGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGAG	9774	0.10183861736207764	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	4050	59.71501	44
TATGCCG	4115	58.77146	46
CTCGTAT	3820	58.5458	42
GTATGCC	4160	58.303967	45
TCGTATG	4185	57.28694	43
ATGCCGT	4500	53.666016	47
TTGTACT	23365	51.435837	1
ACACAGT	4840	50.97999	32
GTACTCT	23970	50.45575	3
CACAGTG	4920	50.222183	33
GCCGTCT	4875	49.39427	49
TACTCTA	24535	49.308105	4
ACAGTGA	4955	48.7378	34
AGTCACA	5100	48.518513	28
TCTAGTT	24920	48.39879	7
CAGTCAC	5270	47.750595	27
CCGTCTT	5065	47.61023	50
CACACAG	5255	47.220387	31
TGTACTC	25800	46.91786	2
CAGTGAT	5120	45.4582	35

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content