H3KCTBGX7_n01_nlp7_3.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3KCTBGX7_n01_nlp7_3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6574990
Sequences flagged as poor quality	0
Sequence length	76
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

$Per base sequence content$

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT	333142	5.066806185256556	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	50045	0.7611418420408244	TruSeq Adapter, Index 3 (100% over 50bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG	48635	0.7396969425048555	No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT	37494	0.5702518178734872	No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG	34448	0.5239247512163516	No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC	32696	0.4972783228567648	No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT	31203	0.4745710639864091	No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG	29829	0.45367369380029476	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	21652	0.32930848564028237	No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT	21345	0.32463927701791184	No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG	20006	0.3042742270330449	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	17735	0.26973425054638867	No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA	16896	0.2569737748650568	No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA	14835	0.22562771958588532	No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT	14675	0.22319425580875407	No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA	13913	0.21160488457016663	No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA	12128	0.18445655430654648	No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG	12083	0.1837721426192283	No Hit
CCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGG	11904	0.18104970501856277	No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA	11595	0.1763500780989781	No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC	11558	0.17578733960051648	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	11352	0.17265425498746007	No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTA	11079	0.16850215741772992	No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA	10959	0.1666770595848815	No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT	10337	0.1572169691512839	No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC	10180	0.15482913281997387	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9434	0.14348310795909955	No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	8813	0.134038226674109	No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCAT	8760	0.1332321417979343	No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT	8262	0.12565798579161336	No Hit
TTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCT	7578	0.1152549281443774	No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA	7423	0.11289751011028153	No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT	7194	0.10941461507926248	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7119	0.10827392893373222	No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA	7049	0.1072092885312373	No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT	6881	0.10465415156524953	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	6300	62.94331	44
TATGCCG	6485	60.877388	46
CTCGTAT	6165	60.745052	42
TCGTATG	6580	60.15849	43
GTATGCC	6655	59.480064	45
ATGCCGT	6845	57.72723	47
TTGTACT	51300	56.42121	1
GCCGTCT	6995	56.289192	49
CCGTCTT	7165	54.855953	50
CATCTCG	6640	54.712868	39
AGTCACT	7330	54.576096	28
TTAGGCA	7285	54.528458	34
TCACTTA	7405	54.165123	30
TCTCGTA	6765	53.908855	41
CAGTCAC	7490	53.45698	27
GTCACTT	7510	53.17481	29
CACTTAG	7550	53.12487	31
GCATCTC	6835	53.100727	38
AAAAAGG	6990	52.57416	69
ACTTAGG	7685	52.236786	32

Summary