FastQCFastQC Report
Tue 3 Jul 2018
H3KCTBGX7_n01_nlp7_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7854805
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT2930203.7304554346034053No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC616130.7843988488574828TruSeq Adapter, Index 2 (97% over 37bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG451220.5744509252616711No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT346050.44055835886441486No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG303280.38610761183759496No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC296600.37760326322550336No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT288360.36711286912915086No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG286070.36419745620674226No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG277160.3528540810370213No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG216490.2756147351843871No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT172500.21961079874038883No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT162520.20690520006543764No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA147530.18782133993141778No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA141260.17983896481198453No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA124480.1584762447953832No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT114630.1459361499107871No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTA110490.14066549074101775No Hit
CCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGG107990.13748272554188168No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCAT107000.1362223505230238No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA101260.1289147216258074No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA98890.12589746021702639No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC97420.12402599427993438No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT96850.12330032381453136No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG90910.11573807370138406No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA89820.11435038807456072No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC86730.11041649028842855No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA84490.10756473267000263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC80500.060.12906344
TATGCCG80350.059.8057346
CTCGTAT80050.058.67448842
GTATGCC83300.057.8978445
TCGTATG84850.056.7576943
ATGCCGT90000.053.47168447
GCCGTCT90200.053.3143249
TTGTACT480400.051.8970681
CCGTCTT94850.050.7743950
TCTCGTA91150.050.6461341
CATCTCG91350.050.22937839
GTACTCT523700.047.8164143
TGTACTC527700.047.4937632
TACATCT96950.047.47244637
TGCCGTC102500.047.12147548
TACTCTA533600.047.112944
AAAAGGG97850.046.7132970
ACTCTAG540500.046.446745
CTATACA103700.046.3399434
CTCTAGT542150.046.22796
Produced by FastQC (version 0.11.5)