H3KCTBGX7_n01_nlp7_33.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3KCTBGX7_n01_nlp7_33.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6481039
Sequences flagged as poor quality	0
Sequence length	76
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT	196793	3.0364421507107116	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC	39399	0.6079117869835376	TruSeq Adapter, Index 7 (97% over 37bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC	31977	0.4933931118143249	No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG	31279	0.4826232337129896	No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT	25610	0.39515269079541104	No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG	25522	0.3937948838141539	No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT	23537	0.3631670786119324	No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG	20586	0.3176342558654561	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	20454	0.3155975453935704	No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG	17580	0.2712528037556941	No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCAT	15262	0.23548693349939726	No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA	13051	0.2013720330953108	No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT	12758	0.1968511530327159	No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA	9935	0.1532933222589773	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	9741	0.1502999750502967	No Hit
CCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGG	9596	0.1480626794561798	No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG	7599	0.1172497187565142	No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA	7537	0.11629308201971937	No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTA	7530	0.11618507464621027	No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC	7500	0.11572218590259988	No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA	7475	0.11533644528292455	No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA	7458	0.11507414166154531	No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT	7188	0.11090814296905172	No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG	7061	0.10894858062110103	No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA	6818	0.10519918179785678	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	4955	58.131638	46
CGTATGC	5085	56.783134	44
CTCGTAT	5010	56.37573	42
GTATGCC	5120	56.258255	45
TCGTATG	5170	55.646473	43
ATGCCGT	5670	50.55459	47
CGGAATA	5820	49.85261	34
GCCGTCT	5810	48.974968	49
TCTCGTA	5915	47.75019	41
TATCTCG	5945	47.50924	39
CAGTCAC	6355	46.096676	27
AGTCACC	6365	45.803955	28
CCGTCTT	6305	45.185493	50
GAATATC	6350	45.14052	36
ACGTCTG	6970	44.79145	15
CCAGTCA	6710	43.449245	26
TCCAGTC	7040	43.003437	25
AATATCT	6790	42.988533	37
CACGTCT	7270	42.94344	14
TGCCGTC	6790	42.47341	48

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content