FastQCFastQC Report
Tue 3 Jul 2018
H3KCTBGX7_n01_nlp7_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12912790
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT345240.2673628239907874No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC281600.21807835487141045TruSeq Adapter, Index 2 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258120.1998948329524448No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA218180.16896425946677673No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG207580.16075534412005463No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT169110.13096317681926214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46600.047.99319546
CGTATGC47550.047.54921744
GTATGCC52050.043.23677445
CTCGTAT53200.039.53886442
TCGTATG57300.039.51946643
GTCACCC65300.034.73120529
TACTCTA83450.034.5204854
GCCGTCT66200.033.4141649
TCTAGTT88250.032.6407367
GTACTCT88950.031.7167843
ACGTCTG75950.030.87462215
TCTCGTA68200.030.79179241
AGTCACC75100.030.38533228
ACTCTAG118600.030.3400925
CACCCAA75800.029.68928731
CTAGTTG95950.029.473468
CAGTCAC77500.029.44436627
GCACACG79400.029.4013111
TCACCCA76900.029.31011430
CCTACTA90200.029.14034834
Produced by FastQC (version 0.11.5)