H3KCTBGX7_n01_nlp7_31.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3KCTBGX7_n01_nlp7_31.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9216788
Sequences flagged as poor quality	0
Sequence length	76
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT	340995	3.6997162135008423	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC	107859	1.1702449920731604	TruSeq Adapter, Index 9 (97% over 36bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG	52127	0.5655657914666151	No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT	44588	0.4837693999254404	No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG	43251	0.46926326177839833	No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC	43060	0.46719095632882085	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	41215	0.44717313667190783	No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT	39257	0.4259292933720511	No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG	36456	0.39553909670049914	No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG	27227	0.29540659934892716	No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT	26222	0.2845025837634543	No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA	19864	0.21551976675605428	No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA	19734	0.21410929707833143	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	17902	0.1942325243891907	No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT	17287	0.18755991783688636	No Hit
CCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGG	16202	0.17578792091127624	No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA	16038	0.1740085591639951	No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA	15247	0.16542639366338902	No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	14821	0.16080439302715868	No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG	14224	0.15432708227638522	No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA	14195	0.1540124390405855	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13867	0.1504537155460232	No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA	13420	0.14560386980800685	No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT	13177	0.14296737648734026	No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC	12124	0.13154257209778503	No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTA	12039	0.13062034192388933	No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC	11220	0.12173438295423525	No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA	10890	0.1181539599261695	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10294	0.11168749894214774	No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC	10021	0.10872551261892971	No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG	9823	0.10657725880209028	No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT	9703	0.10527528679188454	No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	9671	0.10492809425582969	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	13250	61.44012	44
TATGCCG	13340	61.103657	46
CTCGTAT	12780	61.043556	42
TCGTATG	13530	59.78094	43
GTATGCC	13725	59.38996	45
GCCGTCT	14150	57.112095	49
ATGCCGT	14260	57.0397	47
CATTTTA	14580	56.603664	34
TATCTCG	13915	55.28502	39
CAGTCAC	14995	55.06074	27
CCGTCTT	14790	54.593388	50
AGTCACC	15100	54.5391	28
TCTCGTA	14120	54.432495	41
ATTTTAT	15130	53.48194	35
TTATCTC	14605	52.768982	38
ACGTCTG	16140	52.759315	15
CCAGTCA	15800	52.65416	26
TTGTACT	53005	52.39185	1
AAAAGGG	14405	52.04326	70
ACCATTT	15995	51.727497	32

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content