FastQCFastQC Report
Tue 3 Jul 2018
H3KCTBGX7_n01_nlp7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7662746
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT4024895.25254262636397No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG632910.825957169923158No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC492130.6422371301358547No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC484880.6327757699393924TruSeq Adapter, Index 2 (100% over 50bp)
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG468650.6115953732513123No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT457040.5964441467849776No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT428060.558624806303119No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG399670.5215754247889725No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG294340.3841181738243705No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG282230.3683144397582798No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT247800.3233827664390807No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT233400.3045905475661075No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA219050.2858635794531099No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA197130.2572576462798062No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT186850.24384209002882257No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA180420.23545084229596022No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA168350.21969930883785002No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG168320.2196601583818647No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG167210.21821159151040634No Hit
CCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGG161680.21099485745710483No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA158830.20727556413849552No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC152360.19883211579765267No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146460.19113252612053172No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT133650.17441528141478263No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTA128590.16781190450525177No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC128120.16719854736148113No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA126620.16524102456221307No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA125720.16406651088265226No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCAT109460.14284696373858666No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101390.13231549107852458No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA100040.13055372055918335No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT99640.13003171447937856No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC97690.12748693484033008No Hit
TTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCT97090.12670392572062286No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA96670.12615581933682782No Hit
ATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCC95490.12461590140140362No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT92730.12101405945075042No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA90420.11799947433987763No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG88250.11516759135693654No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC87990.1148282874050634No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT87910.11472388618910244No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG83020.10834236186348861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT57900.060.4479842
TATGCCG60250.059.65869546
CGTATGC60650.059.43835444
TCGTATG60400.059.2787543
GTATGCC62950.057.04426645
TTGTACT604050.055.871681
ATGCCGT64750.055.1882147
GCCGTCT66200.053.5571549
CACCGAT67800.053.42895531
GTATCTC65400.052.33886738
TATCTCG66150.051.79802739
CCGTCTT69100.051.76531250
CGATGTA70250.051.76488534
TGTATCT67550.050.82844537
CCGATGT71650.050.65540333
GTACTCT674600.050.2066083
TGTACTC676000.050.1181642
CAGTCAC72450.049.9031527
TCTCGTA69350.049.60978741
ACCGATG73350.049.57681332
Produced by FastQC (version 0.11.5)