FastQCFastQC Report
Tue 3 Jul 2018
H3KCTBGX7_n01_nlp7_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10100911
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1626861.6106072016672555No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC427410.42314005142704453TruSeq Adapter, Index 7 (97% over 35bp)
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG250310.24780933125734894No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG232550.23022675875473014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG232210.22989015545231511No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT201630.19961565842922485No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG196700.19473491054420733No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG178090.17631082978555102No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC163940.16230219234680912No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT162070.1604508741835266No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC158350.15676803805122133No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT156720.15515432221905529No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG152870.1513427848240619No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA145310.14385831139389307No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA141950.1405318787582625No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC140320.13891816292609646No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT139000.13761135010495587No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT130940.12963187181829441No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA130350.12904776608763308No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG130060.12876066327086735No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAA120000.11880116555823528No Hit
GGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGAG111750.11063358542610661No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT109640.10854466493170764No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG109410.10831696269772104No Hit
GGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCT103370.10233730403128986No Hit
CCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAA103080.10205020121452411No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG101600.10058498683930588No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA101580.10056518664504617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG58050.061.9197746
CGTATGC59250.060.90197844
GTATGCC59800.060.34184645
CTCGTAT56250.060.04348442
TCGTATG60450.059.57721343
ACTCAAT65300.053.49019635
GCCGTCT67750.053.36419749
CCACTCA69400.052.14562633
GTACTCT257950.051.7018973
CAGTCAC71150.051.69929527
AGTCACC71800.051.03628528
TACTCTA263650.050.571094
ACCACTC72000.050.45702732
TCTAGTT266000.049.9612127
CACTCAA72200.049.73555834
CTCAATC66950.049.29670736
CCGTCTT73250.048.97508650
CCAGTCA75300.048.4316826
TTGTACT275150.048.3595121
ACGTCTG77350.048.28001415
Produced by FastQC (version 0.11.5)