H3KCTBGX7_n01_nlp7_26.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3KCTBGX7_n01_nlp7_26.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9913715
Sequences flagged as poor quality	0
Sequence length	76
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT	104400	1.0530865573601822	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC	28358	0.28604816660555604	TruSeq Adapter, Index 23 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	21700	0.21888868098386932	No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC	17987	0.18143551635285057	No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG	17205	0.17354745420863923	No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA	16668	0.16813071588198775	No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA	16390	0.1653265198767566	No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG	16010	0.16149344620054137	No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG	15815	0.15952647418248356	No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT	15230	0.15362555812831014	No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG	14983	0.15113406023877024	No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA	14034	0.1415614630842222	No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT	13090	0.1320393011096244	No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT	12873	0.1298504142997857	No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG	12648	0.12758083120202668	No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT	12396	0.12503889813253657	No Hit
CCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAA	12147	0.12252722617101662	No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC	11657	0.11758457853589699	No Hit
GGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGAG	10612	0.10704362592630512	No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG	10561	0.10652918709081309	No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA	10504	0.1059542260393808	No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATG	10414	0.10504639280027718	No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT	10395	0.10485473911646644	No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT	10270	0.10359385961771143	No Hit
CCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAG	10135	0.102232109759056	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	3960	58.420383	46
CGTATGC	4040	57.263546	44
GTATGCC	4195	55.231163	45
TCGTATG	4225	54.838985	43
CTCGTAT	3755	54.805676	42
GTACTCT	17195	50.281693	3
TACTCTA	17650	49.144135	4
TCTAGTT	17785	48.39251	7
GCCGTCT	4815	48.337914	49
AACTAAT	4895	47.047413	35
TAATCTC	4535	45.07109	38
ACGTCTG	5355	44.705315	15
CAGTCAC	5285	44.701195	27
AGTCACC	5335	44.282253	28
TTGTACT	20135	42.787052	1
CACGTCT	5635	42.35971	14
CTAATCT	4895	41.970867	37
CCGTCTT	5585	41.23493	50
CCAGTCA	5715	40.970196	26
TCTCGTA	5015	40.826584	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content