FastQCFastQC Report
Tue 3 Jul 2018
H3KCTBGX7_n01_nlp7_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12050051
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT607760.5043630105797893No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG456140.37853781697687416No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC260280.21599908581299782No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA251160.2084306531150781No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245230.2035095121174176No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG238950.1982979159175343No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT231140.1918166155479342TruSeq Adapter, Index 20 (97% over 44bp)
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC163090.13534382551575924No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA145520.12076297436417488No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC142330.11811568266391569No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT138260.11473810359806777No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATG125370.10404105343620539No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG122060.10129417709518408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC36700.051.87904746
TATGCCG37700.049.9467648
GTATGCC37900.049.86767647
CTCGTAT39300.046.6657144
TCGTATG40900.046.46603845
ATGCCGT40650.046.40818449
GTACTCT115750.045.059433
TACTCTA116450.045.05914
GCCTTAT41500.044.95144737
TCTAGTT118200.044.3871967
TTGTACT122850.041.9449921
GGCCTTA46400.041.18467336
AGTCACG47500.040.4519728
GTCACGT47550.040.18861829
TCACGTG47850.040.15591430
TGTACTC132550.039.3221442
CACGTGG50550.038.08032631
TATCTCG47100.037.7493241
TAGTTGT139300.037.4616979
GCCGTCT50200.037.3703751
Produced by FastQC (version 0.11.5)