FastQCFastQC Report
Tue 3 Jul 2018
H3KCTBGX7_n01_nlp7_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9788138
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG590180.6029543106155635No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT568150.5804474763228716No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC321820.32878572002151996No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG290010.2962871998739699No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA274570.28051300461844736No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT204370.20879354173388237TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG201180.20553449491619347No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC188100.19217138131889844No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC144020.14713727983810607No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA140320.1433571941874951No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG135550.13848394863251826No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA114180.1166513998883138No Hit
GGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGAT111370.11378057808339032No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG109380.11174750499022387No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG109330.1116964227517021No Hit
GGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTG109320.11168620630399775No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG106520.10882560094677865No Hit
CGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG106270.1085701897541698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33100.048.4284748
CGTATGC33200.047.86067646
GTATGCC34700.046.2965647
CTCGTAT35000.043.49930244
TACTCTA112850.043.332494
ATGCCGT37300.042.88134849
TCGTATG37500.042.74598345
TCTAGTT115900.042.4591987
GTACTCT114500.042.4329033
TTGTACT117400.040.940211
ACTCTAG143500.039.5414055
TGTACTC125050.038.8252032
CTAGTTG130850.037.2594348
CTCTAGT132450.037.079086
TAGTTGT131000.037.029759
GCCGTCT42900.036.54952651
CCTACTA128300.035.05399734
ACCTACT128350.034.87655333
TGTTACC137600.034.6428113
GTTACCT138500.034.1651714
Produced by FastQC (version 0.11.5)