FastQCFastQC Report
Tue 3 Jul 2018
H3KCTBGX7_n01_nlp7_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8163957
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG573260.7021840021940341No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT353560.4330743045314913No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC310940.38086922799813866No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG282850.346461893417616No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA260340.3188894796971616No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT253630.31067042611811896TruSeq Adapter, Index 14 (97% over 44bp)
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC194290.23798508492879128No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167440.205096621650506No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC148430.1818113446702377No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA140990.17269811685681344No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG140770.17242863969028746No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG118750.14545642511345908No Hit
GGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGAT116000.14208796053188422No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA112510.13781307275381288No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG111940.1371148819132683No Hit
GGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTG111770.13690664955731638No Hit
CGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG109090.13362392771054526No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG108020.13231328876425977No Hit
GGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGC97250.11912115656660123No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC96740.11849645949874553No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA96660.11839846780182699No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC95620.11712457574188594No Hit
GGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTC94130.11529948038677812No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT91590.11218824400961444No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG90030.11027740591970292No Hit
GTGATGTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGG89270.10934648479897678No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT86120.10548806173280922No Hit
CGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTCGTAGTGGCCG82140.10061297481111181No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG81680.1000495225538302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC36600.054.02861846
TATGCCG36750.053.52304548
GTATGCC37350.052.75694347
CTCGTAT37650.050.01195544
TCGTATG40050.049.28707545
ATGCCGT40650.048.47408749
TCCGTAT40950.047.3497537
CGTATCT40450.045.68537539
CCGTATC41250.045.1387538
TTCCGTA44400.044.4585536
TATCTCG44050.042.1103741
GCCGTCT47550.041.73441751
GTTCCGT48100.041.1841935
TCTCGTA45850.040.45718843
TACTCTA73000.040.1814424
GTCACAG51600.038.66229
TCTAGTT76300.038.624017
AGTTCCG52000.038.2975334
CAGTCAC52650.037.95720727
CACAGTT53100.037.56985531
Produced by FastQC (version 0.11.5)