FastQCFastQC Report
Sat 30 Jun 2018
H3K23BGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3K23BGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12945387
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCCTTACCAGCAGAGATCCAGAAAGGTTAATTAAGGTACCAAAAAA425900.32899750312601705No Hit
ATCGATACCTTGGTGCAGAGATCCAGAATGGTTAATTAAGGTACCAAAAA414920.320515717297598No Hit
ATCGATACCTTGGTGCAGAGATCCAGTATGGTTAATTAAGGTACCAAAAA377870.2918954836962387No Hit
ATCGATACCTTGGTGCAGAGATCCAGTATGGTGAATTAAGGTACCAAAAA304080.23489448403512386No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAATTAAGGTACCAAAAAAT274370.21194422383819042No Hit
ATCGATACCTTGGTGCAGAGATCCAGAATGGTGAATTAAGGTACCAAAAA258260.19949963643419852No Hit
ATCGATACCTTGGTGCAGAGATCCAGTATGGTGAATTAAGGTACCTAAAA252620.1951428721288904No Hit
ATCGATACCTTGGTGCAGAGATCCAGTATGGTTAATTAAGGTACCTAAAA242370.18722499373715132No Hit
ATCGATACCTTGGTGCAGAGATCCACAATGGTTAATTAAGGTACCAAAAA220890.17063221053182884No Hit
TCGATCCTTACCAGCAGAGATCCAGTTAGGTTAATTAAGGTACCAGAAAA202910.1567430931188075No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAATTAAGGTACCAAAAAAG201960.1560092409751829No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAAAAAAT201930.1559860666969632No Hit
TCGATCCTTACCAGCAGAGATCCAGATAGGTTAATTAAGGTACCAAAAAA201290.15549168209494238No Hit
TCGATCGTTACCAGCAGAGATCCAGTTAGGTTAATTAAGGTACCAAAAAA189340.1462605946040856No Hit
TCGATCCTTACCAGCAGAGATCCAGTTAGGTTACTTAAGGTACCAGAAAA183420.14168753703539338No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAATTAAGGTACCAAAAAAC178450.13784833161032575No Hit
ATCGATACCTTGGTGCAGAGATCCAGAATGGGGAATTAAGGTACCAAAAA165510.12785249293821807No Hit
TCGATCCTTACCAGCAGAGATCCAGTTAGGTTAATTAAGGTACCAAAAAA158500.1224374365942092No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAAAAAAG148380.11461998007475559No Hit
ATCGATACCTTGGTGCAGAGATCCAGAATGGTTAATTAAGGTACCTAAAA143150.110579930905117No Hit
CGATCAAGATCGGCAGAGATCCAGTTTCGTTAAGTAAGGTACCAAGAAAT142910.11039453667935921No Hit
ATCGATACCTTGGTGCAGAGATCCAGTATGGGGAATTAAGGTACCAAAAA134720.10406795872537453No Hit
ATCGATACCTTGGTGCAGAGATCCAGTATGGGGAATTAAGGTACCTAAAA131960.10193592512915992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCC1458000.0140.231481
CGATCAA1620100.0140.062381
TCGCGCG1063900.0139.873543
CGATCGC930500.0139.836096
GATCGCG1072450.0139.756131
ATCGCGC1062650.0139.637282
GATCGGC1985200.0139.570478
CGATCCT1427950.0139.509662
ACGATCG935000.0139.13845
CGCGCGG1071450.0139.113394
CGCGGTG1069350.0139.073966
ATACACG952950.0139.031861
TACACGA970100.0138.8142
ATCGGCA1998250.0138.649269
CGGTGCA1255050.0138.634358
TCGCAGA1081250.0138.626229
AGGCAGA3965800.0138.526158
ACACGAT962800.0138.489273
GATCGCA939450.0138.423237
GAGGCAG3356950.0138.39867
Produced by FastQC (version 0.11.5)