FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n02_wt-asynch.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_wt-asynch.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12687540
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8136276.412803427614809No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3081022.4283824917990406Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA233500.063.05039619
ACACGAG234200.062.90553320
CACGAGA246700.060.228621
GAGCACA754000.059.23161316
AGAGCAC771900.059.18731715
CACGTAG178450.059.0061921
ACACGTA178550.057.8366320
GCACAAG227850.057.36910618
AAGAGCA970950.057.3421814
GCACACG471300.056.17563218
AGCACAC532850.056.10541517
GCAGATC284000.054.4749765
AGCACAA245950.053.26270317
ACGAGAT277950.053.0787622
CCAGATC218800.051.4661525
GTGACTG77450.051.171161
AGAGAGA303100.050.5671415
GAGCAAG172950.050.0064916
CACAAGA262750.049.54529619
GAGAGAT306800.049.42051716
Produced by FastQC (version 0.11.5)