FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n02_wt-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_wt-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16757720
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4666412.784632993032465No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC456700.2725311080504985Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGG52150.039.8727879
CGGGCTC45200.036.70429213
TACGGGC48500.034.79097711
GGGCTCG49050.034.10844814
TAGGGGG120350.033.14298638
GGCTCGA50900.032.73112515
CTCGAGT50450.032.67414517
GCACACG75200.032.48416518
AGAGCAC126950.032.3397815
CGGGGGA46200.032.03940649
GAGCACA125700.031.57516516
ACACGAG41400.031.3591920
GCTCGAG54000.031.24072816
CTTAGGG50000.031.21914961
AGCGGGG73250.030.96892410
CCGGAGT54900.029.89337228
CACACGA43450.029.55837419
AGCACAC96500.029.08650417
GTGACTG53750.028.9143311
TTACGGG58650.028.7698610
Produced by FastQC (version 0.11.5)