FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15326976
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG780456850.92046859080356No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1021530.6664915505837551No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG351210.2291450055118505No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN234200.1528024836732308No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG229090.14946849267591988No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC153780.10033290324197024Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG153490.10014369435953968No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGT69300.066.5038719
GCACACG476200.065.4615318
GCACGCG117700.065.2980218
AGAGCAC552500.065.1273115
AGCACAC456450.064.7296617
CGCGCGT85250.064.40549519
ACACGAG151900.064.363820
CACGTAG168100.064.3007721
CACACGA152950.064.2682719
GAGCACA505800.064.17634616
CACGTCA139750.063.94919621
CACACGT311050.063.91296419
ACACGTA157400.062.42598720
AAGAGCA586150.062.0218514
GAGCACG95500.061.82988716
ACACGTC154900.061.8070120
GGCACGC34900.061.5735417
CACGAGA166700.061.10441221
ACGTAGA175500.060.3126722
TTAGCTA69650.060.2912819
Produced by FastQC (version 0.11.5)