FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n02_rad27-exoI-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_rad27-exoI-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13320070
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3939572.9576195920892308No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC583330.43793313398503164Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA145900.041.2619516
ACACGAG44650.041.2239420
AGAGCAC151400.040.85062815
CACACGA45000.040.8270819
CACGAGA49750.037.06788621
GCACACG90400.036.85525518
AGCACAC108700.033.77432317
CACGTAG39100.033.56099321
CAGATCG173800.033.4687776
CGGGCTC30300.032.80802513
GCACAAG67800.032.46772418
AAGAGCA261650.031.98528114
GGGCTCG31650.031.51827614
TACGGGC32100.031.19074811
AGAGATC137100.030.12209917
CTCGAGT34450.029.66431417
CTTAGGG31300.029.18423561
GAGAGAT142050.029.02556616
GCTCGAG35600.028.2168516
GCAGATC75450.028.178835
Produced by FastQC (version 0.11.5)