FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n02_rad27-dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_rad27-dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11909093
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2676422.2473751779417626No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC887960.7456151362660448Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA55950.049.16388319
ACACGAG57000.048.50116720
CACGAGA62000.044.6452921
AGAGCAC192450.042.66773615
GAGCACA189000.042.27897616
CACGTAG48650.039.99289321
GTGACTG51750.038.836071
GCACACG130900.038.82169318
AGCACAC149050.037.64042317
CGGGCTC37450.037.38565413
TGACTGG57200.036.578912
GGGCTCG39150.036.03048314
GACTGGA60650.035.75593
TACGGGC39600.035.6252411
CTCGAGT41100.034.99878317
GCTCGAG41650.034.28668616
GCGGCGC20000.034.1975981
AAGAGCA298600.034.19349314
GCACAAG64850.033.9459618
ACGAGAT81450.033.8980722
Produced by FastQC (version 0.11.5)