FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n02_rad27-asynch.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_rad27-asynch.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11127878
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7026506.314321562475793No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1809911.6264646323405056Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG191500.062.43997220
CACACGA192450.062.04334619
CACGAGA202800.059.8583621
AGAGCAC602400.059.63567715
GAGCACA588200.059.6342916
CACGTAG150350.059.1876921
GCACACG381950.057.8521918
ACACGTA149400.057.619720
AGCACAC428650.057.02117
AAGAGCA749000.056.67373314
GCACAAG171300.055.5282218
ACGAGAT223700.053.35809322
GCAGATC224050.053.322015
ACGTAGA175050.050.7557422
CCAGATC161300.050.3976755
AGCACAA192300.049.44743717
CAGATCG676500.049.3420876
CACACGT202100.048.6049219
GCACGCG26700.046.92383218
GAGAGAT228200.046.7329816
Produced by FastQC (version 0.11.5)