FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n02_rad27-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_rad27-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15264799
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4773023.1268148371950395No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC489160.32044968296012283Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCGC27400.037.5067441
GAGCGGG44000.036.0388229
ACACGAG34800.034.79348420
AGAGCAC117500.034.70406315
GAGCACA115050.034.46875416
CACACGA36950.032.95924819
TAGGGGG99000.032.1612738
CGGGGGA40400.031.35572849
GCACACG66700.030.85285418
CACGAGA41100.030.65210521
AGCGGGG59850.028.65976510
AGCACAC86900.026.86332917
CAGATCG136850.026.4591626
CGGCGCT38600.026.1501942
TAGATCG142400.025.8705776
GGAGAGC50800.024.2649446
CACGTAG32100.023.43867721
GCACAAG64000.023.13152318
CGGCGTT23950.022.6996141
AGGGGTG79650.022.09860228
Produced by FastQC (version 0.11.5)