FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n02_exoI-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_exoI-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12514684
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5346834.272445073323466No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC183060.14627616646173408Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC82000.031.37201715
GAGCACA78700.031.30808316
GAGCGGG26450.030.1747849
TAGGGGG56300.028.33923738
GCACACG44900.028.21549818
CACACGA26850.028.02175519
ACACGAG27250.026.71073520
TAGATCG93850.026.5309526
CAGATCG90500.025.3847456
CGGGGGA28750.024.70904249
AGCACAC60050.024.3611317
CACGAGA31450.023.03191221
GATCGGA333400.022.0838458
AGAGATC81150.022.03771617
AGATCGG333900.022.0110387
TCGGAAG335100.021.60354410
AGCGGGG40300.021.45560510
CGGAAGA347100.020.76556211
ATCGGAA354250.020.6030359
CACGTAG25900.020.40128321
Produced by FastQC (version 0.11.5)