FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n02_dna2-exoI-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_dna2-exoI-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15158613
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4688003.0926312321582454No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC383980.25330813577733Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC128000.032.3204315
GAGCACA126300.031.5356216
CAGATCG186150.031.0197476
ACACGAG37700.030.53883420
CACACGA38800.030.03440519
AGAGATC140950.029.3739317
GTGACTG51000.029.0919911
GCACACG75050.028.67880418
TGACTGG54900.027.8978212
TAGATCG209650.027.4591796
GAGAGAT152350.027.17720416
GACTGGA61150.026.4121253
AGCACAC95150.025.82086217
CACGAGA47300.025.22831721
CGGGCTC25350.025.12854613
GCGGCGC19100.024.4242691
GCAGATC79950.024.0495135
CACGTAG34050.024.04882221
GGGCTCG28000.023.37508614
GATCGGA751100.023.347518
Produced by FastQC (version 0.11.5)