FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n02_dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n02_dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12230949
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3977593.2520698107726558No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC216410.1769363930795558Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG31700.031.13387118
AGAGCAC65500.030.24602115
GAGCACA66350.028.80229216
GAGCGGG26850.028.2907289
TAGGGGG56250.026.4984338
ACACGAG22900.024.75669120
CACACGA22850.024.65811519
AGCACAC49000.023.21334317
CGGGGGA24250.022.65636849
CAGATCG70550.022.3838336
TAGATCG73600.021.5513926
AGCGGGG41350.021.07955210
CACGAGA27900.020.82139621
CGGGGTT18200.020.2335721
GATCGGA268750.019.2009248
AGATCGG271100.018.8937517
TCGGAAG275400.018.64677410
ATCGGAA289450.017.7291979
CGCCGTA103900.017.71650147
AAGAGCA158800.017.69938314
Produced by FastQC (version 0.11.5)