FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n01_wt-asynch.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_wt-asynch.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12687540
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG2876542.2672164974455256TruSeq Adapter, Index 3 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131660.1037711014113059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG90000.044.7628770
ACGTGTG360250.040.3853031
CTTGAAA1531900.039.5600967
TGAAAAA1589950.039.13232869
GAAAAAA1615600.038.8815170
GCTTGAA1552650.038.81093266
TTGAAAA1584300.038.4612468
TGCTTGA1560450.038.17025865
AAAAAGG450850.038.04075670
CTGCTTG1569900.037.93811464
TCTGCTT1628200.036.65176463
CTTCTGC1663500.036.37644261
TTCTGCT1646700.036.15256562
TCTTCTG1730950.034.86231260
GTCTTCT1735050.034.8622359
AAAAAAG542500.032.23352469
CCGTCTT1923800.031.76079657
CGTCTTC1923100.031.73363558
GCCGTCT2020900.030.22424556
CGTGTGC620200.029.6686171
Produced by FastQC (version 0.11.5)