FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n01_wt-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_wt-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16757720
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC5590723.33620564134023TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG414190.24716369529983792TruSeq Adapter, Index 1 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC212820.12699818352377293TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG652550.055.07856470
CTGCTTG833550.054.8148757
TGCTTGA857200.053.27331558
GCTTGAA874600.052.49374859
CCGTCTT877200.052.45644850
TGCCGTC881000.052.43405548
GCCGTCT879850.052.24276449
ATGCCGT892800.051.6983847
TATGCCG897050.051.60423346
CGTCTTC918600.050.1830151
TCTGCTT916800.049.8487156
CACGATC828700.049.83851636
GTATGCC933550.049.65019645
GATCTCG833600.049.5619139
CTTCTGC929250.049.53113654
ACGATCT840800.049.02138537
CGATCTC848500.048.6466538
TCACGAT886000.048.62169335
GTCTTCT949300.048.41631752
CGTATGC959900.048.35274544
Produced by FastQC (version 0.11.5)