FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15326976
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3991842.6044537422124234No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA736600.4805905613736199TruSeq Adapter, Index 14 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG172650.045.343561
TGACTGG189000.043.194692
GACTGGA205900.040.1094323
ATGCCGG62950.039.65375570
TACGTGT36300.039.3517381
ACTGGAG220300.037.69454
TCTCGTT158850.037.2689370
GCTCGTT43700.035.3303370
GACGGGG9400.035.00772
GTTCAGA1201000.034.3322331
AACGTGT56850.033.9337231
TCGTATG3550450.033.92371770
GCGTATG352650.033.73417370
CACGTGT50650.033.52531
CTGGAGT250100.033.5112845
GCTCGTG24900.032.33843670
CTCGTAT3645400.031.08756369
GGAGTTC275900.030.6589877
CGCGTAT345850.030.42918669
TGGAGTT280750.030.2769666
Produced by FastQC (version 0.11.5)