FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n01_triple-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_triple-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13266463
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1165020.8781692603371373TruSeq Adapter, Index 8 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG238800.039.0169858
TGCCGTC260250.038.5307349
TATGCCG270900.038.25772547
ATGCCGT266850.038.12963548
GCCGTCT242900.037.83922250
GTATGCC283900.037.14628246
CCGTCTT257050.036.69495451
TGCTTGA262950.036.0856659
GCTTGAA285300.036.01880360
CGTATGC294150.035.16172445
CTCGTAT275800.034.7344543
GTCACAC393800.033.6818530
ATCTCGT280450.033.5955341
CGTCTTC279500.033.38439652
TCGTATG290150.033.22165744
AATCTCG295350.033.1667840
GAATCTC313550.031.8779539
AGTCACA418100.031.81621629
CAGTCAC420000.031.66323728
TCTGCTT301600.031.47312757
Produced by FastQC (version 0.11.5)