FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n01_rad27-exoI-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_rad27-exoI-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13320070
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG590890.44360877983373964TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG82700.024.430991
ATGCCGG27650.024.0507570
CGTGTGC110550.021.6337951
CTGCTTG233800.019.5368358
GTGTGCT128800.018.7842482
TGCTTGA254750.018.17741659
GCTTGAA264250.017.80174660
TATACGG45350.016.43858162
CCGATCT274450.015.7813656
TCTGCTT295100.015.73975657
TCCGATC276450.015.6798615
TAACTAG39350.015.56411330
CTTCTGC297900.015.497992555
CTTGAAA311700.015.48428361
TACGGTG47100.015.30804664
CTTCCGA287300.015.20929053
ATACGGT51150.014.8482863
TCTTCCG301150.014.7656472
ACGGTGT49700.014.71860465
CCGTCTT316450.014.621984551
Produced by FastQC (version 0.11.5)