FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n01_rad27-dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_rad27-dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11909093
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG720860.6053021838019067TruSeq Adapter, Index 5 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG37850.025.05950270
ACGTGTG127350.024.9672761
CTGCTTG255450.023.30637658
GACGTGT83650.022.5216711
GCTTGAA287300.021.86734260
TGCTTGA274150.021.79313959
CGTGTGC178100.021.450881
AAAAAGG233550.021.40017970
GTGTGCT195450.019.525992
TCTGCTT311650.019.33977757
CTTCTGC318100.018.81598755
CTTGAAA342150.018.79119361
CCGTCTT348100.017.76656251
TTCTGCT328700.017.76151556
GCCGTCT351950.017.41331750
GTCTTCT342800.017.36774853
TGCCGTC372600.017.09633649
CGTCTTC368550.016.79023252
TCTTCTG357900.016.60654
TGTGCTC231900.016.5323183
Produced by FastQC (version 0.11.5)